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NEN Life Science ecl plustm western blotting detection system
Ecl Plustm Western Blotting Detection System, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plustm/ecl+western+blotting+detection+system/pm39074903-249-7-13
Average 90 stars, based on 1 article reviews
ecl plustm western blotting detection system - by Bioz Stars, 2026-09
90/100 stars

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Article Title: TcrPDEA1, a cAMP-specific phosphodiesterase with atypical pharmacological properties from Trypanosoma cruzi.
Article Snippet: Cyclic nucleotide phosphodiesterases (PDEs) catalyze the degradation of cAMP and cGMP, and regulate a variety of cellular processes by ontrolling the levels of these second messengers.. We have previously described the presence of both a calcium-stimulated adenylyl cyclase nd two membrane-bound cAMP-specific PDEs (one of them strongly associated to the flagellum and the other one with a possible vesicular ocalization) in Trypanosoma cruzi.. Here we report the identification and characterization of TcrPDEA1, a singular phosphodiesterase of T. cruzi hich is resistant to the typical phosphodiesterase inhibitors, such as IBMX, papaverine and theofylline.

Article Title: TcPDE4, a novel membrane-associated cAMP-specific phosphodiesterase from Trypanosoma cruzi.
Article Snippet: Cyclic nucleotide phosphodiesterases constitute the only known mechanism to inactivate regulatory signals involving cAMP or cGMP.. In our laboratory a cAMP-specific phosphodiesterase associated to the flagellar apparatus, named TcPDE1, was identified in Trypanosoma cruzi.. By using the catalytic domain sequence of TcPDE1 to screen a Trypanosoma cruzi genomic data base, a novel T. cruzi phosphodiesterase sequence w s p c c f f s f p ©

Western Blot:

Article Title: Defining the role of a FYVE domain in the localization and activity of a cAMP phosphodiesterase implicated in osmoregulation in Trypanosoma cruzi
Article Snippet: .. The latter was developed with the ECL PlusTM Western Blotting Detection System (NEN Life Science Products Inc., Boston, MA). .. The intensity of the bands obtained was quantified using the Gene Tools Software of Syngene images capture systems.

Article Title: The NADPH-cytochrome P450 reductase family in Trypanosoma cruzi is involved in the sterol biosynthesis pathway.
Article Snippet: Trypanosoma cruzi flavoproteins TcCPR-A, TcCPR-B and TcCPR-C are members of the NADPH-dependent cytochrome P-450 reductase family expressed in the parasite.. Epimastigotes over-expressing TcCPR-B and TcCPR-C showed enhanced ergosterol biosynthesis and increased NADP/NADPH ratio.. Transgenic parasites with augmented ergosterol content presented a higher membrane order with a corresponding diminished bulk-phase endocytosis.

Article Title: Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
Article Snippet: For TcVps34, the transferred membranes were incubated for 2 h with a 1:4,000 dilution of rat anti-HA high affinity monoclonal antibody (Roche Applied Science), and detection was carried out by incubating with a 1:5,000 dilution of a goat anti-rabbit or goat anti-rat conjugated to peroxidase (Sigma-Aldrich). .. The membranes were then developed with the ECL PlusTM Western Blotting Detection System (NEN Life Science Products). .. To control for sample loading, the blots were also probed with a monoclonal mouse anti–β-tubulin antibody (1:400 dilution; Chemicon International).

Article Title: Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes.
Article Snippet: Accepted Manuscript Title: Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes Author: Alba Marina Gimenez Marı́a Celeste Gesumarı́a Alejandra C. Schoijet Guillermo D. Alonso Mirtha M. Flawiá Graciela E. Racagni Estela E. Machado PII: S0166-6851(15)30033-5 DOI: http://dx.doi.org/doi:10.1016/j.molbiopara.2015.10.002 Reference: MOLBIO 10931 To appear in: Molecular & Biochemical Parasitology Received date: 10-4-2015 Revised date: 9-10-2015 Accepted date: 13-10-2015 Please cite this article as: Gimenez Alba Marina, Gesumaría María Celeste, Schoijet Alejandra C, Alonso Guillermo D, Flawiá Mirtha M, Racagni Graciela E, Machado Estela E.Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes.Molecular and Biochemical Parasitology http://dx.doi.org/10.1016/j.molbiopara.2015.10.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Multiple NADPH-cytochrome P450 reductases from Trypanosoma cruzi suggested role on drug resistance.
Article Snippet: rotein ostag s. Cy ins su quenc TcCP .. Dedu r FM whic d in nden iodon Article history: Received 27 December 2007 Received in revised form 12 March 2008 Accepted 13 March 2008 Available online 21 March 2008

Article Title: Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi .
Article Snippet: For TcVps34, the transferred membranes were incubated for 2 h with a 1:4,000 dilution of rat anti-HA high affinity monoclonal antibody (Roche Applied Science), and detection was carried out by incubating with a 1:5,000 dilution of a goat anti-rabbit or goat antirat conjugated to peroxidase (Sigma-Aldrich). .. The membranes were then developed with the ECL PlusTM Western Blotting Detection System (NEN Life Science Products). .. To control for sample loading, the blots were also probed with a monoclonal mouse anti–β-tubulin antibody (1:400 dilution; Chemicon International).



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